dsh-molbio-tools

Wet-lab grad students and postdocs juggle a dozen point tools every day: SnapGene for plasmid maps, Primer3 for primer design, an online GC/Tm calculator, manual restriction-site checklists, a spreadsheet for delta-delta-Ct. Context bounces across browser tabs and the experimental workflow keeps breaking. dsh-molbio-tools drops 44 deterministic, zero-dependency molecular-biology tools directly into a DeepSeek Harness conversation, so a single natural-language prompt can drive reverse-complement, six-frame translation, 90-plus restriction-enzyme site scanning (including IIS-type cut geometries), automatic primer design with Primer3-aligned structural screening (self-complementarity, hairpins, dimers, 3-prime stability, mismatch tolerance, target-position bias), restriction-ligation / Gibson / Golden Gate assembly simulation, circular-aware Sanger verification with .ab1 quality values, intron-spanning qPCR primer design, delta-delta-Ct and standard-curve analysis, progressive multi-sequence alignment with conservation scoring (v15), codon optimization, protein property estimation, one-click SVG plasmid maps from SnapGene .dna or GenBank files, a virtual agarose gel, and literature / protocol / experiment log management.

Every tool is a pure, deterministic function returning structured JSON. Generated graphics are written to the workspace and auto-opened in the OS default viewer, keeping the model context slim. The plugin ships as a dedicated preset profile (Molecular Biology Lab), so its 44 tools appear only in that mode and never leak into unrelated sessions. Built entirely from .mjs files with no build step and no runtime dependencies, it is ideal for research groups and independent labs that want to compress the entire molecular-biology analysis pipeline into a single conversational workflow.